Development and validation of a sensitive LC-MS/MS method for pioglitazone: application towards pharmacokinetic and tissue distribution study in rats.

ABSTRACT: In the present study, a sensitive LC-MS/MS method was developed and validated to measure pioglitazone (PGZ) concentrations in rat plasma and tissues. The chromatographic separation was achieved by using a YMC Pro C₁₈ column (100 mm × 4.6 mm, 3μ) with a mobile phase consisting of formic acid (0.1% v/v) and acetonitrile (5 : 95) at a flow rate of 0.7 mL min^-1 and injection volume of 10 μL (IS: rosiglitazone). Mass spectrometric detection was done using triple quadrupole mass spectrometry using the ESI interface operating in a positive ionization mode. The developed method was validated over a linearity range of 1-500 ng mL^-1 with detection and a lower quantification limit of 0.5 ng mL^-1 and 1 ng mL^-1. The method accuracy ranged from 95.8

SUBMITTER: G KK 

PROVIDER: S-EPMC8695949 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature