Single-Domain Antibodies for Targeting, Detection, and In Vivo Imaging of Human CD4⁺ Cells.

ABSTRACT: The advancement of new immunotherapies necessitates appropriate probes to monitor the presence and distribution of distinct immune cell populations. Considering the key role of CD4⁺ cells in regulating immunological processes, we generated novel single-domain antibodies [nanobodies (Nbs)] that specifically recognize human CD4. After in-depth analysis of their binding properties, recognized epitopes, and effects on T-cell proliferation, activation, and cytokine release, we selected CD4-specific Nbs that did not interfere with crucial T-cell processes in vitro and converted them into immune tracers for noninvasive molecular imaging. By optical imaging, we demonstrated the ability of a high-affinity CD4-Nb to specifically visualize CD4⁺ cells in vivo using

SUBMITTER: Traenkle B 

PROVIDER: S-EPMC8696186 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature