ITRAQ-based proteomic and physiological analyses of broccoli sprouts in response to exogenous melatonin with ZnSO₄ stress.

ABSTRACT: Exogenous melatonin (10 μM) enhances ZnSO₄ (4 mM) stress tolerance and regulates the isothiocyanate content of broccoli sprouts. Nevertheless, the molecular mechanism underlying the role of melatonin in isothiocyanate metabolism under ZnSO₄ stress is unclear. The effects of exogenous melatonin on growth and isothiocyanate metabolism in broccoli sprouts under ZnSO₄ stress during germination were investigated by physio-biochemical methods, quantification of relative gene expression levels, and the isobaric tags for the relative and absolute quantitation (iTRAQ) labelling technique. Compared with sprouts under ZnSO₄ stress alone, sprout length, fresh weight and free calcium content increased significantly in sprouts under ZnSO₄ stress plu

SUBMITTER: Yin Y 

PROVIDER: S-EPMC8696995 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature