Localization atomic force microscopy.

ABSTRACT: Understanding structural dynamics of biomolecules at the single-molecule level is vital to advancing our knowledge of molecular mechanisms. Currently, there are few techniques that can capture dynamics at the sub-nanometre scale and in physiologically relevant conditions. Atomic force microscopy (AFM)¹ has the advantage of analysing unlabelled single molecules in physiological buffer and at ambient temperature and pressure, but its resolution limits the assessment of conformational details of biomolecules². Here we present localization AFM (LAFM), a technique developed to overcome current resolution limitations. By applying localization image reconstruction algorithms³ to peak positions in high-speed AFM and conventional AFM data, we increase the resolution

SUBMITTER: Heath GR 

PROVIDER: S-EPMC8697813 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature