Voluntary wheel running behaviour as a tool to assess the severity in a mouse pancreatic cancer model.

ABSTRACT: Laboratory animals frequently undergo routine experimental procedures such as handling, restraining and injections. However, as a known source of stress, these procedures potentially impact study outcome and data quality. In the present study, we, therefore, performed an evidence-based severity assessment of experimental procedures used in a pancreatic cancer model including surgical tumour induction and subsequent chemotherapeutic treatment via repeated intraperitoneal injections. Cancer cell injection into the pancreas was performed during a laparotomy under general anaesthesia. After a four-day recovery phase, mice received either drug treatment (galloflavin and metformin) or the respective vehicle substances via daily intraperitoneal injections. In addition to clinical scoring, an auto

SUBMITTER: Weegh N 

PROVIDER: S-EPMC8699632 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature