Evaluation of Reference Genes for Transcriptional Profiling in Two Cockroach Models.

ABSTRACT: The German cockroach, Blattella germanica, and the American cockroach, Periplaneta americana are the most common and synanthropic household pests of interest to public health. While they have increasingly served as model systems in hemimetabolous insects for studying many biological issues, there is still a lack of stable reference gene evaluation for reliable quantitative real-time PCR (qPCR) outputs and functional genomics. Here, we evaluated the expression variation of common insect reference genes, including the historically used actin, across various tissues and developmental stages, and also under experimental treatment conditions in these two species by using three individual algorithms (geNorm, BestKeeper, and NormFinder) and a comprehensive program (RefFinder)

SUBMITTER: Zhu S 

PROVIDER: S-EPMC8701133 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature