Expression patterns of long non-coding RNAs in peripheral blood mononuclear cells of non-segmental vitiligo.

ABSTRACT:

Objective

We explored the patterns of long non-coding RNA (lncRNA) expression in peripheral blood mononuclear cells (PBMCs) from patients with non-segmental vitiligo.

Methods

We used high-throughput RNA sequencing technology to generate sequence data from five patients with non-segmental vitiligo alongside five normal healthy individuals, and then performed bioinformatics analyses to detect the differential expression of lncRNA in PBMCs. Gene Ontology (GO) and pathway analyses were performed for functional annotation, and quantitative real-time polymerase chain reaction (qRT-PCR) was used to verify gene expression. Target miRNAs and mRNAs of differentially expressed lncRNAs were predicted using bioinformatics analysis.

Results

A total of 292 lncRNAs were differentiall

SUBMITTER: Zhang S 

PROVIDER: S-EPMC8702240 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature