Proteogenomic Analysis Reveals Proteins Involved in the First Step of Adipogenesis in Human Adipose-Derived Stem Cells.

ABSTRACT:

Background

Obesity is characterized as a disease that directly affects the whole-body metabolism and is associated with excess fat mass and several related comorbidities. Dynamics of adipocyte hypertrophy and hyperplasia play an important role in health and disease, especially in obesity. Human adipose-derived stem cells (hASC) represent an important source for understanding the entire adipogenic differentiation process. However, little is known about the triggering step of adipogenesis in hASC. Here, we performed a proteogenomic approach for understanding the protein abundance alterations during the initiation of the adipogenic differentiation process.

Methods

hASC were isolated from adipose tissue of three donors and were then characterized and expanded. Cells were culture

SUBMITTER: Bonilauri B 

PROVIDER: S-EPMC8702357 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature