Bile Acids Gate Dopamine Transporter Mediated Currents.

ABSTRACT: Bile acids (BAs) are molecules derived from cholesterol that are involved in dietary fat absorption. New evidence supports an additional role for BAs as regulators of brain function. Sterols such as cholesterol interact with monoamine transporters, including the dopamine (DA) transporter (DAT) which plays a key role in DA neurotransmission and reward. This study explores the interactions of the BA, obeticholic acid (OCA), with DAT and characterizes the regulation of DAT activity via both electrophysiology and molecular modeling. We expressed murine DAT (mDAT) in Xenopus laevis oocytes and confirmed its functionality. Next, we showed that OCA promotes a DAT-mediated inward current that is Na⁺-dependent and not regulated by intracellular calcium. The current induced

SUBMITTER: Romanazzi T 

PROVIDER: S-EPMC8702627 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature