Next-Generation Sequencing for Pathogen Identification in Infected Foot Ulcers.

ABSTRACT:

Background

Accurate identification of primary pathogens in foot infections remains challenging due to the diverse microbiome. Conventional culture may show false-positive or false-negative growth, leading to ineffective postoperative antibiotic treatment. Next-generation sequencing (NGS) has been explored as an alternative to standard culture in orthopedic infections. NGS is highly sensitive and can detect an entire bacterial genome along with genes conferring antibiotic resistance in a given sample. We investigated the potential use of NGS for accurate identification and quantification of microbes in infected diabetic foot ulcer (DFU). We hypothesize that NGS will aid identification of dominant pathogen and provide a more complete profile of microorganisms in infected DFUs compare

SUBMITTER: Choi Y 

PROVIDER: S-EPMC8702686 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature