Assessment of Aspartate and Bicarbonate Produced From Hyperpolarized [1-¹³C]Pyruvate as Markers of Renal Gluconeogenesis.

ABSTRACT: As both a consumer and producer of glucose, the kidney plays a significant role in glucose homeostasis. Measuring renal gluconeogenesis requires invasive techniques, and less invasive methods would allow renal gluconeogenesis to be measured more routinely. Magnetic resonance spectroscopy and imaging of infused substrates bearing hyperpolarized carbon-13 spin labels allows metabolism to be detected within the body with excellent sensitivity. Conversion of hyperpolarized 1-¹³C pyruvate in the fasted rat liver is associated with gluconeogenic flux through phosphoenolpyruvate carboxykinase (PEPCK) rather than pyruvate dehydrogenase (PDH), and this study tested whether this was also the case in the kidney. The left kidney was scanned in fed and overnight-fasted rats either with or wi

SUBMITTER: Yoshihara HAI 

PROVIDER: S-EPMC8702956 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature