Designing Red-Shifted Molecular Emitters Based on the Annulated Locked GFP Chromophore Derivatives.

ABSTRACT: Bioimaging techniques require development of a wide variety of fluorescent probes that absorb and emit red light. One way to shift absorption and emission of a chromophore to longer wavelengths is to modify its chemical structure by adding polycyclic aromatic hydrocarbon (PAH) fragments, thus increasing the conjugation length of a molecule while maintaining its rigidity. Here, we consider four novel classes of conformationally locked Green Fluorescent Protein (GFP) chromophore derivatives obtained by extending their aromatic systems in different directions. Using high-level ab initio quantum chemistry calculations, we show that the alteration of their electronic structure upon annulation may unexpectedly result in a drastic change of their fluorescent properties. A flip of optically bright

SUBMITTER: Sinenko GD 

PROVIDER: S-EPMC8703576 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature