Dataset Information

Molecular Authentication, Phytochemical Evaluation and Asexual Propagation of Wild-Growing Rosa canina L. (Rosaceae) Genotypes of Northern Greece for Sustainable Exploitation.

ABSTRACT: Dogroses belong to a taxonomically difficult genus and family and represent important phytogenetic resources associated with high ornamental, pharmaceutical-cosmetic and nutritional values, thus suggesting a potentially high exploitation merit. Triggered by these prospects, wild-growing Rosa canina populations of Greece were selected for investigation and evaluation of their potential for integrated domestication. We collected ripe rosehips from Greek native wild-growing populations (samples from seven genotypes) for phytochemical analysis (total phenolics, total flavonoids, antioxidant activity and vitamin C content), leaf samples for DNA analysis using the ITS2 sequence (nine genotypes) and fresh soft-wood stem cuttings for propagation trials (seven genotypes). After evaluation of these materials, this study reports for the first-time distinct DNA-fingerprinted genotypes from Greece with interesting phytochemical profiles mainly in terms of Vitamic C content (up to 500.22 ± 0.15 mg of ascorbic acid equivalents/100 g of sample) as well as effective asexual propagation protocols for prioritized R. canina genotypes via cuttings. The latter highlights the importance of the levels of external hormone application (2000 ppm of indole-3-butyric acid), the effect of season (highly-effective spring trials) and genotype-specific differences in rooting capacities of the studied genotypes. All inclusive, this study offers new artificially selected material of Greek native R. canina with a consolidated identity and interesting phytochemical profile. These materials are currently under ex-situ conservation for further evaluation and characterization in pilot field studies, thus facilitating its sustainable exploitation for applications in the agro-alimentary, medicinal-cosmetic, and ornamental sectors.

SUBMITTER: Maloupa E

PROVIDER: S-EPMC8704562 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature

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Publications

Molecular Authentication, Phytochemical Evaluation and Asexual Propagation of Wild-Growing <i>Rosa canina</i> L. (Rosaceae) Genotypes of Northern Greece for Sustainable Exploitation.

Maloupa Eleni E Karapatzak Eleftherios E Ganopoulos Ioannis I Karydas Antonis A Papanastasi Katerina K Kyrkas Dimitris D Yfanti Paraskevi P Nikisianis Nikos N Zahariadis Anthimos A Kosma Ioanna S IS Badeka Anastasia V AV Patakioutas Giorgos G Fotakis Dimitrios D Krigas Nikos N

Plants (Basel, Switzerland) 20211130 12

Dogroses belong to a taxonomically difficult genus and family and represent important phytogenetic resources associated with high ornamental, pharmaceutical-cosmetic and nutritional values, thus suggesting a potentially high exploitation merit. Triggered by these prospects, wild-growing <i>Rosa canina</i> populations of Greece were selected for investigation and evaluation of their potential for integrated domestication. We collected ripe rosehips from Greek native wild-growing populations (samp ...[more]

PMID: 34961105

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Project description:ObjectiveThe aromatic profile of Rosa canina L. petals hold immense potential for the fragrance and pharmaceutical industries. This study aims to investigate the chemical composition and gene expression patterns across different floral development stages to uncover the biosynthetic pathways of floral scent.MethodsEssential oils (EOs) were extracted from petals at five developmental stages (S1-S5) and analyzed using Gas Chromatography-Mass Spectrometry (GC-MS), identifying 20 distinct compounds. RNA isolation and quantitative real-time PCR (qRT-PCR) analysis were performed to assess gene expression.ResultsStage S3, notable for its enhanced aromatic profile, was dominated by terpenoid compounds such as β-Citronellol (1.18%), Caryophyllene (8.59%), β-Selinene (1.50%), and Caryophyllene oxide (0.50%), indicating significant upregulation of terpenoid biosynthesis genes. qRT-PCR analysis revealed that CCD1 had the highest expression in S4 (9.51-fold), while DXR and DXS peaked at S3 with fold changes of 29058.38 and 73.35, respectively. Other genes like AAT1, LIS, and GPS also showed peak expressions at S3 with fold changes of 1.33, 10.70, and 1.18, respectively. PAR exhibited the highest expression in S1, while GGPPS peaked in S4 (2.01-fold). Clustering analysis indicated distinct groupings of developmental stages and gene expression patterns, with strong correlations between specific genes and compounds, such as CCD1 with GGPPS (0.78) and β-Citronellol with Caryophyllene (0.92). Principal Component Analysis (PCA) highlighted significant contributions of AAT1, GPS, and nonadecane compounds to the overall variance.ConclusionThese findings provide a comprehensive understanding of the chemical and genetic factors shaping the aromatic profile of R. canina, with promising applications for both the fragrance and pharmaceutical sectors. The study's innovation lies in the detailed correlation between EO composition and gene expression, presenting new insights into the biosynthetic pathways of floral scent.

Dataset Information

Molecular Authentication, Phytochemical Evaluation and Asexual Propagation of Wild-Growing Rosa canina L. (Rosaceae) Genotypes of Northern Greece for Sustainable Exploitation.

Publications

Molecular Authentication, Phytochemical Evaluation and Asexual Propagation of Wild-Growing <i>Rosa canina</i> L. (Rosaceae) Genotypes of Northern Greece for Sustainable Exploitation.

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