Method of Microglial DNA-RNA Purification from a Single Brain of an Adult Mouse.

ABSTRACT: Microglia, the resident brain immune effectors cells, show dynamic activation level changes for most neuropsychiatric diseases, reflecting their complex regulatory function and potential as a therapeutic target. Emerging single-cell molecular biology studies are used to investigate the genetic modification of individual cells to better understand complex gene regulatory pathways. Although multiple protocols for microglia isolation from adult mice are available, it is always challenging to get sufficient purified microglia from a single brain for simultaneous DNA and RNA extraction for subsequent downstream analysis. Moreover, for data comparison between treated and untreated groups, standardized cell isolation techniques are essential to decrease variability. Here, we present a combined me

SUBMITTER: Raihan MO 

PROVIDER: S-EPMC8704779 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature