Ion Conductance-Based Perfusability Assay of Vascular Vessel Models in Microfluidic Devices.

ABSTRACT: We present a novel methodology based on ion conductance to evaluate the perfusability of vascular vessels in microfluidic devices without microscopic imaging. The devices consisted of five channels, with the center channel filled with fibrin/collagen gel containing human umbilical vein endothelial cells (HUVECs). Fibroblasts were cultured in the other channels to improve the vascular network formation. To form vessel structures bridging the center channel, HUVEC monolayers were prepared on both side walls of the gel. During the culture, the HUVECs migrated from the monolayer and connected to the HUVECs in the gel, and vascular vessels formed, resulting in successful perfusion between the channels after culturing for 3-5 d. To evaluate perfusion without microscopic imaging, Ag/AgCl wires we

SUBMITTER: Akasaka R 

PROVIDER: S-EPMC8705798 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature