Identification of Bull Semen Microbiome by 16S Sequencing and Possible Relationships with Fertility.

ABSTRACT: Reports on the use of 16S sequencing for the identification of bacteria in healthy animals are lacking. Bacterial contamination of bull semen can have a negative effect on the sperm quality. The aims of this study were threefold: to identify bacteria in the semen of healthy bulls using 16S sequencing; to investigate the differences in the bacterial community between individual bulls; and to establish if there was a relationship between the bacteria isolated and bull fertility. Semen from 18 bulls of known fertility was used for the DNA extraction and 16S sequencing; 107 bacterial genera were identified. The differences in the amplicon sequence variants (ASVs) and the numbers of genera between bulls were noted. Negative correlations (p < 0.05) between several bacterial genera with Curvibact

SUBMITTER: Cojkic A 

PROVIDER: S-EPMC8705814 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature