Experimental Characterization of the Interaction between the N-Terminal SH3 Domain of Crkl and C3G.

ABSTRACT: Crkl is a protein involved in the onset of several cancer pathologies that exerts its function only through its protein-protein interaction domains, a SH2 domain and two SH3 domains. SH3 domains are small protein interaction modules that mediate the binding and recognition of proline-rich sequences. One of the main physiological interactors of Crkl is C3G (also known as RAPGEF1), an interaction with key implications in regulating cellular growth and differentiation, cell morphogenesis and adhesion processes. Thus, understanding the interaction between Crkl and C3G is fundamental to gaining information about the molecular determinants of the several cancer pathologies in which these proteins are involved. In this paper, through a combination of fast kinetics at different experimental condit

SUBMITTER: Pagano L 

PROVIDER: S-EPMC8705818 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature