Modeling Transposition of the Great Arteries with Patient-Specific Induced Pluripotent Stem Cells.

ABSTRACT: The dextro-transposition of the great arteries (d-TGA) is one of the most common congenital heart diseases. To identify biological processes that could be related to the development of d-TGA, we established induced pluripotent stem cell (iPSC) lines from two patients with d-TGA and from two healthy subjects (as controls) and differentiated them into endothelial cells (iPSC-ECs). iPSC-EC transcriptome profiling and bioinformatics analysis revealed differences in the expression level of genes involved in circulatory system and animal organ development. iPSC-ECs from patients with d-TGA showed impaired ability to develop tubular structures in an in vitro capillary-like tube formation assay, and interactome studies revealed downregulation of biological processes related to Notch signaling, cir

SUBMITTER: Ontoria-Oviedo I 

PROVIDER: S-EPMC8705900 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature