Environment-Sensitive Fluorescent Labelling of Peptides by Luciferin Analogues.

ABSTRACT: Environment-sensitive fluorophores are very valuable tools in the study of molecular and cellular processes. When used to label proteins and peptides, they allow for the monitoring of even small variations in the local microenvironment, thus acting as reporters of conformational variations and binding events. Luciferin and aminoluciferin, well known substrates of firefly luciferase, are environment-sensitive fluorophores with unusual and still-unexploited properties. Both fluorophores show strong solvatochromism. Moreover, luciferin fluorescence is influenced by pH and water abundance. These features allow to detect local variations of pH, solvent polarity and local water concentration, even when they occur simultaneously, by analyzing excitation and emission spectra. Here, we describe the

SUBMITTER: Siepi M 

PROVIDER: S-EPMC8706149 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature