A Novel Flow Cytometric Approach for the Quantification and Quality Control of Chlamydia trachomatis Preparations.

ABSTRACT: Chlamydia trachomatis is an obligate intracellular pathogenic bacterium with a biphasic developmental cycle manifesting two distinct morphological forms: infectious elementary bodies (EBs) and replicative intracellular reticulate bodies (RBs). Current standard protocols for quantification of the isolates assess infectious particles by titering inclusion-forming units, using permissive cell lines, and analyzing via immunofluorescence. Enumeration of total particle counts is achieved by counting labeled EBs/RBs using a fluorescence microscope. Both methods are time-consuming with a high risk of observer bias. For a better assessment of C. trachomatis preparations, we developed a simple and time-saving flow cytometry-based workflow for quantifying small particles, such as EBs wi

SUBMITTER: Klasinc R 

PROVIDER: S-EPMC8706156 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature