Biostimulation of Bacteria in Liquid Culture for Identification of New Antimicrobial Compounds.

ABSTRACT: We hypothesized that environmental microbiomes contain a wide range of bacteria that produce yet uncharacterized antimicrobial compounds (AMCs) that can potentially be used to control pathogens. Over 600 bacterial strains were isolated from soil and food compost samples, and 68 biocontrol bacteria with antimicrobial activity were chosen for further studies based on inhibition assays against a wide range of food and plant pathogens. For further characterization of the bioactive compounds, a new method was established that used living pathogens in a liquid culture to stimulate bacteria to produce high amounts of AMCs in bacterial supernatants. A peptide gel electrophoresis microbial inhibition assay was used to concurrently achieve size separation of the antimicrobial peptides. Fifteen poten

SUBMITTER: Mirzaee H 

PROVIDER: S-EPMC8706287 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature