Insights into the Neutralization and DNA Binding of Toxin-Antitoxin System ParE_SO-CopA_SO by Structure-Function Studies.

ABSTRACT: ParE_SO-CopA_SO is a new type II toxin-antitoxin (TA) system in prophage CP4So that plays an essential role in circular CP4So maintenance after the excision in Shewanella oneidensis. The toxin ParE_SO severely inhibits cell growth, while CopA_SO functions as an antitoxin to neutralize ParE_SO toxicity through direct interactions. However, the molecular mechanism of the neutralization and autoregulation of the TA operon transcription remains elusive. In this study, we determined the crystal structure of a ParE_SO-CopA_SO complex that adopted an open V-shaped heterotetramer with the organization of ParE_SO-(CopA_SO)₂-ParE_SO. The structure showed that upon ParE_SO b

SUBMITTER: Zhou J 

PROVIDER: S-EPMC8706911 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature