A Cell Culture System to Investigate Marek's Disease Virus Integration into Host Chromosomes.

ABSTRACT: Marek's disease virus (MDV) is a highly oncogenic alphaherpesvirus that causes a devastating neoplastic disease in chickens. MDV has been shown to integrate its genome into the telomeres of latently infected and tumor cells, which is crucial for efficient tumor formation. Telomeric repeat arrays present at the ends of the MDV genome facilitate this integration into host telomeres; however, the integration mechanism remains poorly understood. Until now, MDV integration could only be investigated qualitatively upon infection of chickens. To shed further light on the integration mechanism, we established a quantitative integration assay using chicken T cell lines, the target cells for MDV latency and transformation. We optimized the infection conditions and assessed the establishment of laten

SUBMITTER: You Y 

PROVIDER: S-EPMC8706938 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature