Minimizing Cholesterol-Induced Aggregation of Membrane-Interacting DNA Origami Nanostructures.

ABSTRACT: DNA nanotechnology provides methods for building custom membrane-interacting nanostructures with diverse functions, such as shaping membranes, tethering defined numbers of membrane proteins, and transmembrane nanopores. The modification of DNA nanostructures with hydrophobic groups, such as cholesterol, is required to facilitate membrane interactions. However, cholesterol-induced aggregation of DNA origami nanostructures remains a challenge. Aggregation can result in reduced assembly yield, defective structures, and the inhibition of membrane interaction. Here, we quantify the assembly yield of two cholesterol-modified DNA origami nanostructures: a 2D DNA origami tile (DOT) and a 3D DNA origami barrel (DOB), by gel electrophoresis. We found that the DOT assembly yield (relative to the no c

SUBMITTER: Daljit Singh JK 

PROVIDER: S-EPMC8707602 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature