Population Variability Generated during Rescue Process and Passaging of Recombinant Mumps Viruses.

ABSTRACT: Recombinant mumps viruses (MuVs) based on established vaccine strains represent attractive vector candidates as they have known track records for high efficacy and the viral genome does not integrate in the host cells. We developed a rescue system based on the consensus sequence of the L-Zagreb vaccine and generated seven different recombinant MuVs by (a) insertion of one or two additional transcription units (ATUs), (b) lengthening of a noncoding region to the extent that the longest noncoding region in MuV genome is created, or (c) replacement of original L-Zagreb sequences with sequences rich in CG and AT dinucleotides. All viruses were successfully rescued and faithfully matched sequences of input plasmids. In primary rescued stocks, low percentages of heterogeneous positions were foun

SUBMITTER: Slovic A 

PROVIDER: S-EPMC8707793 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature