Development of an Indirect ELISA Based on Spike Protein to Detect Antibodies against Feline Coronavirus.

ABSTRACT: Feline coronavirus (FCoV) is a pathogenic virus commonly found in cats that causes a benign enteric illness and fatal systemic disease, feline infectious peritonitis. The development of serological diagnostic tools for FCoV is helpful for clinical diagnosis and epidemiological investigation. Therefore, this study aimed to develop an indirect enzyme-linked immunosorbent assay (iELISA) to detect antibodies against FCoV using histidine-tagged recombinant spike protein. FCoV S protein (1127-1400 aa) was expressed and used as an antigen to establish an ELISA. Mice and rabbits immunized with the protein produced antibodies that were recognized and bound to the protein. The intra-assay coefficient of variation (CV) was 1.15-5.04% and the inter-assay CV was 4.28-15.13%, suggesting an acceptable re

SUBMITTER: Dong B 

PROVIDER: S-EPMC8707903 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature