Coupling/Uncoupling Reversibility in Isolated Mitochondria from Saccharomyces cerevisiae.

ABSTRACT: The yeast Saccharomyces cerevisiae uses fermentation as the preferred pathway to obtain ATP and requires the respiratory chain to re-oxidize the NADH needed for activity of Glyceraldehyde-3-phosphate. This process is favored by uncoupling of oxidative phosphorylation (OxPhos), which is at least partially controlled by the mitochondrial unspecific pore (_ScMUC). When mitochondrial ATP synthesis is needed as in the diauxic phase or during mating, a large rise in Ca²⁺ concentration ([Ca²⁺]) closes _ScMUC, coupling OxPhos. In addition, _ScMUC opening/closing is mediated by the ATP/ADP ratio, which indicates cellular energy needs. Here, opening and closing of _ScMUC was evaluated in isolated mitocho

SUBMITTER: Morales-Garcia L 

PROVIDER: S-EPMC8707985 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature