Efficient Decellularization by Application of Moderate High Hydrostatic Pressure with Supercooling Pretreatment.

ABSTRACT: Decellularized tissues are considered superior scaffolds for cell cultures, preserving the microstructure of native tissues and delivering many kinds of cytokines. High hydrostatic pressure (HHP) treatment could remove cells physically from biological tissues rather than chemical methods. However, there are some risks of inducing destruction or denaturation of extracellular matrices (ECMs) at an ultrahigh level of HHP. Therefore, efficient decellularization using moderate HHP is required to remove almost all cells simultaneously to suppress tissue damage. In this study, we proposed a novel decellularization method using a moderate HHP with supercooling pretreatment. To validate the decellularization method, a supercooling device was developed to incubate human dermal fibroblasts or collage

SUBMITTER: Zemmyo D 

PROVIDER: S-EPMC8708072 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature