The Promotion of Genomic Instability in Human Fibroblasts by Adenovirus 12 Early Region 1B 55K Protein in the Absence of Viral Infection.

ABSTRACT: The adenovirus 12 early region 1B55K (Ad12E1B55K) protein has long been known to cause non-random damage to chromosomes 1 and 17 in human cells. These sites, referred to as Ad12 modification sites, have marked similarities to classic fragile sites. In the present report we have investigated the effects of Ad12E1B55K on the cellular DNA damage response and on DNA replication, considering our increased understanding of the pathways involved. We have compared human skin fibroblasts expressing Ad12E1B55K (55K⁺HSF), but no other viral proteins, with the parental cells. Appreciable chromosomal damage was observed in 55K⁺HSFs compared to parental cells. Similarly, an increased number of micronuclei was observed in 55K⁺HSFs, both in cycling cells and after DNA dama

SUBMITTER: Abualfaraj T 

PROVIDER: S-EPMC8708088 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature