Structural Diversity, Fitness Cost, and Stability of a Bla_NDM-1-Bearing Cointegrate Plasmid in Klebsiella pneumoniae and Escherichia coli.

ABSTRACT: Cointegrate/hybrid plasmids combine the genetic elements of two or more plasmids and generally carry abundant antimicrobial resistance determinants. Hence, the spread of cointegrate plasmids will accelerate the transmission of AMR genes. To evaluate the transmission risk caused by cointegrate plasmids, we investigated the structural diversity, fitness cost, and stability of a cointegrate plasmid in Klebsiella pneumoniae YZ6 and Escherichia coli EC600. The cointegrate plasmid pSL131_IncA/C_IncX3 was from a clinical Salmonella Lomita strain. After transferring the plasmid into E. coli EC600 by conjugation, we observed plasmids with different structures, including a full-length original plasmid and two truncated versions. By contrast, DNA fragment deletion and b

SUBMITTER: Liu Z 

PROVIDER: S-EPMC8708245 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature