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Characterization of Ictalurid herpesvirus 1 Glycoprotein ORF59 and Its Potential Role on Virus Entry into the Host Cells.

ABSTRACT: The channel catfish virus (CCV, Ictalurid herpesvirus 1) has caused sustained economic losses in the fish industry because of its strong infectivity and pathogenicity. Thus, it is necessary to determine the function of viral proteins in the CCV infection process. The present study aimed to characterize CCV glycoprotein ORF59 and explore its impact on virus infection in host cells. Firstly, its exclusive presence in the membrane fraction of the cell lysate and subcellular localization verified that CCV ORF59 is a viral membrane protein expressed at late-stage infection. A protein blocking assay using purified His6 tagged ORF59, expressed in sf9 insect cells using a baculovirus expression system, indicated a dose-dependent inhibitory effect of recombinant ORF59 protein on virus invasion. Knockdown of the ORF59 using a short hairpin (shRNA) showed that ORF59 silencing decreased the production of infectious virus particles in channel catfish ovary cells. The results of this study suggest that recombinant ORF59 protein might inhibit CCV entry into the host cells. These findings will promote future studies of the key functions of glycoprotein ORF59 during CCV infection.

SUBMITTER: Li SX

PROVIDER: S-EPMC8709185 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature

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Characterization of <i>Ictalurid herpesvirus</i> 1 Glycoprotein ORF59 and Its Potential Role on Virus Entry into the Host Cells.

Li Shu-Xin SX Yu Fei F Chen Hong-Xun HX Zhang Xiao-Dong XD Meng Li-Hui LH Hao Kai K Zhao Zhe Z

Viruses 20211129 12

The channel catfish virus (CCV, <i>Ictalurid herpesvirus</i> 1) has caused sustained economic losses in the fish industry because of its strong infectivity and pathogenicity. Thus, it is necessary to determine the function of viral proteins in the CCV infection process. The present study aimed to characterize CCV glycoprotein ORF59 and explore its impact on virus infection in host cells. Firstly, its exclusive presence in the membrane fraction of the cell lysate and subcellular localization veri ...[more]

PMID: 34960662

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Dataset Information

Characterization of Ictalurid herpesvirus 1 Glycoprotein ORF59 and Its Potential Role on Virus Entry into the Host Cells.

Publications

Characterization of <i>Ictalurid herpesvirus</i> 1 Glycoprotein ORF59 and Its Potential Role on Virus Entry into the Host Cells.

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