Isolation and Characterization of A2-EPTX-Nsm1a, a Secretory Phospholipase A₂ from Malaysian Spitting Cobra (Naja sumatrana) Venom.

ABSTRACT: Phospholipase A₂ (PLA₂) toxins are one of the main toxin families found in snake venom. PLA₂ toxins are associated with various detrimental effects, including neurotoxicity, myotoxicity, hemostatic disturbances, nephrotoxicity, edema, and inflammation. Although Naja sumatrana venom contains substantial quantities of PLA₂ components_, there is limited information on the function and activities of PLA₂ toxins from the venom. In this study, a secretory PLA₂ from the venom of Malaysian N. sumatrana, subsequently named A2-EPTX-Nsm1a, was isolated, purified, and characterized. A2-EPTX-Nsm1a was purified using a mass spectrometry-guided approach and multiple chromatography steps. Based on LC-MSMS, A2-EPTX-Ns

SUBMITTER: Abdullah NAH 

PROVIDER: S-EPMC8709200 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature