Transcriptome analysis of transgenic apple fruit overexpressing microRNA172 reveals candidate transcription factors regulating apple fruit development at early stages.

ABSTRACT:

Background

MicroRNA172 (miR172) has been proven to be critical for fruit growth, since elevated miR172 activity blocks the growth of apple (Malus x domestica Borkh.) fruit. However, it is not clear how overexpression of miR172 affects apple fruit developmental processes.

Methods

To answer this question, the present study, analyzed global transcriptional changes in miR172-overexpressing (miR172OX) and nongenetically modified wild-type (WT) apple fruit at two developmental stages and in different fruit tissues via RNA-seq. In addition, two cultivars, 'Hanfu' and 'M9', which have naturally fruit size variation, were included to identify miR172-dependent DEGs. qRT-PCRwas used to verify the reliability of our RNA-seq data.

Results

Overexpression of miR172 altered th

SUBMITTER: Zhou Z 

PROVIDER: S-EPMC8710058 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature