Dataset Information

A diguanylate cyclase regulates biofilm formation in Rhodococcus sp. NJ-530 from Antarctica.

ABSTRACT: Biofilms represent a protective survival mode in which bacteria adapt themselves to the natural environment for survival purposes. Biofilm formation is regulated by 3,5-cyclic diguanylic acid (c-di-GMP), which is a universal second messenger molecule in bacteria. Diguanylate cyclase (DGC) catalyses c-di-GMP intracellular synthesis, which plays important roles in bacterial adaptation to the natural environment. In this study, the DGC gene was first cloned from Antarctic Rhodococcus sp. NJ-530. DGC contained 948 nucleotides and encoded 315 amino acids with a molecular weight of 34.6 KDa and an isoelectric point of 5.58. qRT-PCR demonstrated that the DGC expression level was significantly affected by lower salinity and temperature. Consistently, more biofilm formation occurred under the same stress. It has been shown that Rhodococcus sp. NJ-530 can adapt to the extreme environment in Antarctica, which is closely related to biofilm formation. These results provide an important reference for studying the adaptive mechanism of Antarctic microorganisms to this extreme environment.

Supplementary information

The online version contains supplementary material available at 10.1007/s13205-021-03093-z.

SUBMITTER: Wang X

PROVIDER: S-EPMC8710177 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

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A diguanylate cyclase regulates biofilm formation in Rhodococcus sp. NJ-530 from Antarctica.

Wang Xixi X He Yingying Y Deng Yashan Y Zuo Zhicong Z Li Dan D Chen Fushan F Qu Changfeng C Miao Jinlai J

3 Biotech 20211225 1

Biofilms represent a protective survival mode in which bacteria adapt themselves to the natural environment for survival purposes. Biofilm formation is regulated by 3,5-cyclic diguanylic acid (c-di-GMP), which is a universal second messenger molecule in bacteria. Diguanylate cyclase (DGC) catalyses c-di-GMP intracellular synthesis, which plays important roles in bacterial adaptation to the natural environment. In this study, the DGC gene was first cloned from Antarctic Rhodococcus ...[more]

PMID: 35036275

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Dataset Information

A diguanylate cyclase regulates biofilm formation in Rhodococcus sp. NJ-530 from Antarctica.

Supplementary information

Publications

A diguanylate cyclase regulates biofilm formation in <i>Rhodococcus</i> sp. NJ-530 from Antarctica.

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