Development of a Quantitative Real-Time PCR Assay for Detection of Toxoplasma gondii in Brain Samples.

ABSTRACT:

Background

Toxoplasmosis is a worldwide-distributed infection that can cause serious diseases, mainly in congenitally infected and immunodeficient individuals. PCR assays play an indispensable role in the detection of Toxoplasma gondii in different biological samples.

Methods

This study was conducted in the Parasitology Department at Pasteur Institute of Iran (Tehran) during 2016-2018. We designed a highly sensitive quantitative real-time PCR (RT-qPCR) targeted REP-529, a noncoding repetitive DNA. We cloned the amplicon in a plasmid (pTZREP-529) and used it to generate the standard curve. The Toxoplasma RT-qPCR characteristics, i.e., detection limit, specificity, linear dynamic range, linearity, intra-, and inter-assay precisions, were determined. The detection limit

SUBMITTER: Berizi M 

PROVIDER: S-EPMC8710198 | biostudies-literature | 2021 Oct-Dec

REPOSITORIES: biostudies-literature