A Round Trip to the Desert: In situ Nanopore Sequencing Informs Targeted Bioprospecting.

ABSTRACT: Bioprospecting expeditions are often performed in remote locations, in order to access previously unexplored samples. Nevertheless, the actual potential of those samples is only assessed once scientists are back in the laboratory, where a time-consuming screening must take place. This work evaluates the suitability of using Nanopore sequencing during a journey to the Tabernas Desert (Spain) for forecasting the potential of specific samples in terms of bacterial diversity and prevalence of radiation- and desiccation-resistant taxa, which were the target of the bioprospecting activities. Samples collected during the first day were analyzed through 16S rRNA gene sequencing using a mobile laboratory. Results enabled the identification of locations showing the greatest and the least potential,

SUBMITTER: Latorre-Perez A 

PROVIDER: S-EPMC8710813 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature