ERF9 of Poncirus trifoliata (L.) Raf. undergoes feedback regulation by ethylene and modulates cold tolerance via regulating a glutathione S-transferase U17 gene.

ABSTRACT: Plant ethylene-responsive factors (ERFs) play essential roles in cold stress response, but the molecular mechanisms underlying this process remain poorly understood. In this study, we characterized PtrERF9 from trifoliate orange (Poncirus trifoliata (L.) Raf.), a cold-hardy plant. PtrERF9 was up-regulated by cold in an ethylene-dependent manner. Overexpression of PtrERF9 conferred prominently enhanced freezing tolerance, which was drastically impaired when PtrERF9 was knocked down by virus-induced gene silencing. Global transcriptome profiling indicated that silencing of PtrERF9 resulted in substantial transcriptional reprogramming of stress-responsive genes involved in different biological processes. PtrERF9 was further verified to directly and specifically bind with the promoters of glut

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC8710834 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature