Production of aromatic three-line hybrid rice using novel alleles of BADH2.

ABSTRACT: Aroma is a key grain quality trait that directly influences the market price of rice globally. Loss of function of betaine aldehyde dehydrogenase 2 (OsBADH2) affects the biosynthesis of 2-acetyl-1-pyrroline (2-AP), which is responsible for aroma in fragrant rice. The current study was aimed at creating new alleles of BADH2 using CRISPR/Cas9 gene editing technology under the genetic background of the japonica Ningjing 1 (NJ1) and indica Huang Huazhan (HHZ) varieties. Sensory evaluation and analysis using headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) showed that the grains of the four homozygous T₁ lines with new alleles of BADH2 (nj1-cr ^BADH2 -1, nj1-cr ^BADH2 -2, hhz-cr ^BADH2 -1 and hhz-cr ^BADH2

SUBMITTER: Hui S 

PROVIDER: S-EPMC8710899 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature