Effects of Gelatin Methacrylate Hydrogel on Corneal Repair and Regeneration in Rats.

ABSTRACT:

Purpose

This study investigates the repairing process of rat cornea after surgery of lamellar keratoplasty (LKP) and evaluates the effects of gelatin methacrylate (GelMA) hydrogel.

Methods

In the LKP group, the lamellar stroma matrixes of Sprague-Dawley rats were transplanted to enhanced green fluorescent protein rats, whereas those in the GelMA group were also embedded with a GelMA hydrogel during the corneal transplantation. Grafted eyes were harvested on days seven, 30, and 90. Hematoxylin and eosin staining, immunofluorescence staining, scanning electron microscopy, optical coherence tomography, and a slit-lamp microscope were used to study the process of corneal restoration and regeneration.

Results

A total of 42 rats were analyzed, including 18 rats in each of t

SUBMITTER: Chen Y 

PROVIDER: S-EPMC8711000 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature