Trehalose: A mycogenic cell wall elicitor elicit resistance against leaf spot disease of broccoli and acts as a plant growth regulator

ABSTRACT: Highlights • Mycogenic cell wall elicitor was isolated from trichoderma atroviride.• The isolated elicitor was identified as trehalose by LC-MS analysis.• Seed priming with elicitor enhanced early germination and vigour.• Primed plants induced resistance against leaf spot disease of brocolli.• Trehalose sugar act as a bio-stimulant for growth promotion and plant defence. Elicitors are biochemicals, and the cell wall-derived elicitors from fungi can trigger defence mechanisms in plants by increasing the phytoalexin accumulation when they encounter the pathogens. The main objective of this research was to purify and characterize a cell wall elicitor from Trichoderma atroviride (TaCWE) and evaluate the seed priming effect of that elicitor for inducing systemic resistance in broccoli plants against leaf spot disease. Amongst the tested TaCWE concentrations of the seed priming (5, 10, & 25 mg ml−1), 10.0 mg ml−1 showed significantly (P < 0.05) improved early emergence, the rate of germination at 94%, and observed seedling vigour of 2601. Also, elicitor (10 mg ml−1) treatment alone induced 57% plant protection. On the contrary, the elicitor treated and pathogen inoculated plants induced a notable 72% protection against leaf spot disease of broccoli caused by A. brassicicola. Thus, the primed seeds with elicitor showed induced disease resistance and plant growth promotion. The prominent molecule present in the purified extracted cell wall elicitor is confirmed as trehalose. The AFM analysis indicated the trehalose length and width as 10.16 µm and 2.148 µm, respectively. FTIR chromatogram further confirmed trehalose in abundance with traces of carbon, hydrogen, nitrogen, oxygen, and LC-MS profile with a single peak eluted with a retention time of 3.78 min. The findings of this study contribute to understanding better the role of trehalose, a biogenic cell-wall elicitor that can induce systemic resistance against leaf spot disease and regulate plant growth in the broccoli plants.

SUBMITTER: De Britto S 

PROVIDER: S-EPMC8711064 | biostudies-literature |

REPOSITORIES: biostudies-literature