Tissue fixation effects on human retinal lipid analysis by MALDI imaging and LC-MS/MS technologies.

ABSTRACT: Imaging mass spectrometry (IMS) allows the location and abundance of lipids to be mapped across tissue sections of human retina. For reproducible and accurate information, sample preparation methods need to be optimized. Paraformaldehyde fixation of a delicate multilayer structure like human retina facilitates the preservation of tissue morphology by forming methylene bridge crosslinks between formaldehyde and amine/thiols in biomolecules; however, retina sections analyzed by IMS are typically fresh-frozen. To determine if clinically significant inferences could be reliably based on fixed tissue, we evaluated the effect of fixation on analyte detection, spatial localization, and introduction of artifactual signals. Hence, we assessed the molecular identity of lipids generated by matrix-ass

SUBMITTER: Kotnala A 

PROVIDER: S-EPMC8711642 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature