Fms-like tyrosine kinase 3 is a regulator of the cardiac side population in mice.

ABSTRACT: Fms-like tyrosine kinase 3 (Flt3) is a regulator of hematopoietic progenitor cells and a target of tyrosine kinase inhibitors. Flt3-targeting tyrosine kinase inhibitors can have cardiovascular side effects. Flt3 and its ligand (Flt3L) are expressed in the heart, but little is known about their physiological functions. Here, we show that cardiac side population progenitor cells (SP-CPCs) from mice produce and are responsive to Flt3L. Compared with wild-type, flt3L^-/- mice have less SP-CPCs with less contribution of CD45^-CD34⁺ cells and lower expression of genes related to epithelial-to-mesenchymal transition, cardiovascular development and stem cell differentiation. Upon culturing, flt3L^-/- SP-CPCs show increased proliferation and less vasculogeni

SUBMITTER: Della Verde G 

PROVIDER: S-EPMC8711848 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature