Achieving maximum efficiency of Mungbean yellow mosaic India virus infection in mungbean by agroinoculation.

ABSTRACT: Mungbean is one of the important food legumes in the Indian-sub-continent. Yellow mosaic disease, caused by Mungbean yellow mosaic virus and Mungbean yellow mosaic India virus (MYMIV) poses a severe threat to its production. Agroinoculation has been the most preferred way to test the function of genomic components of these viruses. However, the available inoculation methods are not as efficient as whitefly transmission, thereby limiting their usage for screening and biological studies. We hereby report an efficient and reproducible agroinoculation method for achieving maximum (100%) efficiency using tandem repeat infectious agro-constructs of DNA A and DNA B of MYMIV. The present study targeted wounding of various meristematic tissues of root, shoot, parts of germinating seeds and also non

SUBMITTER: Sivalingam PN 

PROVIDER: S-EPMC8712281 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature