WAY-100635 Alleviates Corneal Lesions Through 5-HT_1A Receptor-ROS-Autophagy Axis in Dry Eye.

ABSTRACT: Purpose: To explore whether 5-HT_1A receptors are involved in the dry eye disease (DED) mouse model and reveal its underlying mechanism. Methods: A C57BL/6J mouse DED model was established via the administration of 0.2% benzalkonium chloride twice a day for 14 days. Corneal fluorescein sodium staining score and Schirmer I test were checked before, and on days 7, 14, and 21 after treatment. The experiment was randomly divided into control, DED, 5-HT_1A receptor agonist with or without N-acetylcysteine (NAC) and 5-HT_1A receptor antagonist with or without NAC groups. The mRNA expression of inflammatory cytokines was measured by reverse transcription-quantitative polymerase chain reaction. Cellular reactive oxygen species (ROS) were detected by 2',

SUBMITTER: Zhou X 

PROVIDER: S-EPMC8712493 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature