Multiplex Droplet Digital Polymerase Chain Reaction Assay for Rapid Molecular Detection of Pathogens in Patients With Sepsis: Protocol for an Assay Development Study.

ABSTRACT:

Background

Blood cultures are the cornerstone of diagnosis for detecting the presence of bacteria or fungi in the blood, with an average detection time of 48 hours and failure to detect a pathogen occurring in approximately 50% of patients with sepsis. Rapid diagnosis would facilitate earlier treatment and/or an earlier switch to narrow-spectrum antibiotics.

Objective

The aim of this study is to develop and implement a multiplex droplet digital polymerase chain reaction (ddPCR) assay as a routine diagnostic tool in the detection and identification of pathogens from whole blood and/or blood culture after 3 hours of incubation.

Methods

The study consists of three phases: (1) design of primer-probe pairs for accurate and reliable quantification of the most common sepsis-

SUBMITTER: Badran S 

PROVIDER: S-EPMC8713102 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature