Electrostatics Drive Oligomerization and Aggregation of Human Interferon Alpha-2a.

ABSTRACT: Aggregation is a common phenomenon in the field of protein therapeutics and can lead to function loss or immunogenic patient responses. Two strategies are currently used to reduce aggregation: (1) finding a suitable formulation, which is labor-intensive and requires large protein quantities, or (2) engineering the protein, which requires extensive knowledge about the protein aggregation pathway. We present a biophysical characterization of the oligomerization and aggregation processes by Interferon alpha-2a (IFNα-2a), a protein drug with antiviral and immunomodulatory properties. This study combines experimental high throughput screening with detailed investigations by small-angle X-ray scattering and analytical ultracentrifugation. Metropolis Monte Carlo simulations are used to gain insig

SUBMITTER: Pohl C 

PROVIDER: S-EPMC8713289 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature