Circulating tumor DNA methylation marker MYO1-G for diagnosis and monitoring of colorectal cancer.

ABSTRACT:

Background

Circulating tumor DNA (ctDNA) is a promising diagnostic and prognostic marker for many cancers and has been actively investigated in recent years. Previous studies have already demonstrated the potential use of ctDNA methylation markers in the diagnosis and prognostication of colorectal cancer (CRC). This retrospective study validated the value of methylation biomarker MYO1-G (cg10673833) in CRC diagnosis and disease monitoring using digital droplet PCR (ddPCR), a biomarker selected from our previous study due to its highest diagnostic efficiency.

Methods

Blood samples of CRC and control samples from tumor-free individuals at two institutions were collected to quantify the methylation ratio using ddPCR. Area under curve (AUC) was calculated after constructing rece

SUBMITTER: Lin WH 

PROVIDER: S-EPMC8713401 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature