Transcriptome-Wide Off-Target Effects of Steric-Blocking Oligonucleotides.

ABSTRACT: Steric-blocking oligonucleotides (SBOs) are short, single-stranded nucleic acids designed to modulate gene expression by binding to RNA transcripts and blocking access from cellular machinery such as splicing factors. SBOs have the potential to bind to near-complementary sites in the transcriptome, causing off-target effects. In this study, we used RNA-seq to evaluate the off-target differential splicing events of 81 SBOs and differential expression events of 46 SBOs. Our results suggest that differential splicing events are predominantly hybridization driven, whereas differential expression events are more common and driven by other mechanisms (including spurious experimental variation). We further evaluated the performance of in silico screens for off-target splicing events, and f

SUBMITTER: Holgersen EM 

PROVIDER: S-EPMC8713556 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature