Low-energy electron holography imaging of conformational variability of single-antibody molecules from electrospray ion beam deposition.

ABSTRACT: Imaging of proteins at the single-molecule level can reveal conformational variability, which is essential for the understanding of biomolecules. To this end, a biologically relevant state of the sample must be retained during both sample preparation and imaging. Native electrospray ionization (ESI) can transfer even the largest protein complexes into the gas phase while preserving their stoichiometry and overall shape. High-resolution imaging of protein structures following native ESI is thus of fundamental interest for establishing the relation between gas phase and solution structure. Taking advantage of low-energy electron holography's (LEEH) unique capability of imaging individual proteins with subnanometer resolution, we investigate the conformational flexibility of Herceptin, a mono

SUBMITTER: Ochner H 

PROVIDER: S-EPMC8713884 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature