Biofidelic dynamic compression of human cortical spheroids reproduces neurotrauma phenotypes.

ABSTRACT: Fundamental questions about patient heterogeneity and human-specific pathophysiology currently obstruct progress towards a therapy for traumatic brain injury (TBI). Human in vitro models have the potential to address these questions. Three-dimensional spheroidal cell culture protocols for human-origin neural cells have several important advantages over their two-dimensional monolayer counterparts. Three-dimensional spheroidal cultures may mature more quickly, develop more biofidelic electrophysiological activity and/or reproduce some aspects of brain architecture. Here, we present the first human in vitro model of non-penetrating TBI employing three-dimensional spheroidal cultures. We used a custom-built device to traumatize these spheroids in a quantifiable, repeatable and biofidelic mann

SUBMITTER: Shoemaker AR 

PROVIDER: S-EPMC8713991 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature